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. Author manuscript; available in PMC: 2006 Oct 5.
Published in final edited form as: Chromosoma. 2004 May 25;112(8):398–409. doi: 10.1007/s00412-004-0285-5

Fig. 6a-k.

Fig. 6a-k

Overexpression of GFP-SMN (green fluorescent protein-” survival of motor neurons protein product) in UR61 cells does not induce gem formation. Both undifferentiated (a-c) and differentiated (d-k) UR61 cells were transiently transfected with GFP-SMN and stained for coilin. GFP-SMN and coilin colocalize in all Cajal bodies (CBs) of transfected cells, whereas gems are not visible. Differentiated UR61 cells with low (d-f), moderate (j) and high (g-i) expression levels of cytoplasmic SMN show the same staining pattern of their nuclear bodies. k Numerous GFP-SMN aggregates appear distributed throughout the cytoplasm and within a long neurite in a differentiated UR61 cell. Bar represents 5 μm