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. Author manuscript; available in PMC: 2007 Jun 1.
Published in final edited form as: Protein Expr Purif. 2005 Dec 27;47(2):571–590. doi: 10.1016/j.pep.2005.11.026

Fig. 4.

Fig. 4

GnGn hydrolysis by SfGlcNAcase-1 and -3. The synthetic oligosaccharide substrate GnGn was incubated for about 16 h with microsomal fractions containing 40 ug of total protein from Sf9 cells infected with (B) wild type baculovirus, (C) AcGlcNAcase-1, or (D) AcGlcNAcase-3. The reaction products were then recovered and analyzed by reverse-phase HPLC, as described in Materials and Methods. (A) Elution of untreated synthetic oligosaccharides MGn, MM, GnGn and GnM, from left to right. The structures are shown in standard oligosaccharide drawing format [5] on the right.