Fig. 7.
Hydrolysis of GnM by SfGlcNAcase-1 and -3. GnM was incubated for about 16 h with microsomal fractions containing 40 ug of total protein from Sf9 cells infected with (B) wild type baculovirus, (C) AcGlcNAcase-1, or (D) AcGlcNAcase-3. The reaction products were then recovered and analyzed by reverse-phase HPLC, as described in Materials and Methods. (A) Elution of untreated synthetic oligosaccharides and their structures, as in Figure 4.