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. Author manuscript; available in PMC: 2007 Jun 1.
Published in final edited form as: Protein Expr Purif. 2005 Dec 27;47(2):571–590. doi: 10.1016/j.pep.2005.11.026

Fig. 8.

Fig. 8

Hydrolysis of chitotriose by SfGlcNAcase-1 and -3. Chitotriose was incubated for about 16 h with microsomal fractions containing 40 ug of total protein from Sf9 cells infected with (B) wild type baculovirus, (C) AcGlcNAcase-1, or (D) AcGlcNAcase-3. The reaction products were then recovered and analyzed by reverse-phase HPLC, as described in Materials and Methods. (A) Elution of chitotriose. Elution position of monomeric N-acetylglucosamine was determined in a parallel HPLC analysis.