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. Author manuscript; available in PMC: 2007 Jun 1.
Published in final edited form as: Protein Expr Purif. 2005 Dec 27;47(2):571–590. doi: 10.1016/j.pep.2005.11.026

Fig. 9.

Fig. 9

Nature of SfGlcNAcase-1 and -3 membrane association. Microsomal fractions containing 40 ug of total protein from Sf9 cells infected with (B) wild type baculovirus, (C) AcGlcNAcase-1, or (D) AcGlcNAcase-3 were subjected to two rounds of dilution, sonication, and centrifugation, as described in Materials and Methods. The microsomal pellets were then solubilized, incubated for about 16 h with GnGn, and the reaction products were recovered and analyzed by reverse-phase HPLC, as described in Materials and Methods. (A) Elution of untreated synthetic oligosaccharides and their structures, as in Figure 4.