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. 2006 Oct 6;2(10):e164. doi: 10.1371/journal.pgen.0020164

Figure 2. Deletions by Reversed Ac Ends Transposition Generate Chimerical Genes.

Figure 2

The solid circle indicates the centromere, the short vertical line indicates the target site, and the other symbols have the same meaning as those in Figure 1. (For animated version, see Video S1).

(A) Ac transposase (blue oval) binds to the 5′ end of Ac and 3′ end of fAc.

(B) As in ordinary transposition, the Ac 5′ end and the fAc 3′ end are excised by transposase cleavage, and the sequences flanking the Ac/fAc ends join together to form a ~13-kb circle. The X mark at the junction indicates the transposon footprint.

(C) The excised transposon ends insert into a site in intron 2 of p2. The Ac 5′ end joins to the distal side of the insertion site to form a circle, and the fAc 3′ end joins to the proximal side of the insertion site to generate a chimeric gene containing exon 1 and exon 2 of p2 and exon 3 of p1.

This study reports the isolation of the progenitor (A) and deletion products (C). Note that the hypothetical structures shown in (B) are transient in nature and would not be amenable to physical isolation.