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. 2006 Oct 6;2(10):e164. doi: 10.1371/journal.pgen.0020164

Figure 3. RT-PCR Analysis of P-oo Transcripts.

Figure 3

RNA was extracted from kernel pericarp (20 DAP), reverse transcribed, and PCR-amplified using primers complementary to both p1 and p2 transcripts. The progenitor allele (P1-rr11) shows amplification of a 605-bp band from p1. The p-ww2 and P-oo alleles show amplification of a 522-bp band characteristic of the 5′ region of the p2 gene. The p1-ww1112 allele has a deletion of p1; the native p2 gene is intact in this allele, but is not expressed in kernel pericarp.