(A and B) Whole-cell S-type anion channel current traces in wild-type (WT) guard cells in the absence of ABA (A) and in the presence of 50 μM ABA (B).
(C and D) Whole-cell S-type anion channel current traces in cpk3-1cpk6–1 double mutant guard cells in the absence of ABA (C) and in the presence of ABA (D).
(E) Average current-voltage curves of wild-type and cpk3-1cpk6–1 and cpk3-2cpk6–2 double mutant guard cells in the absence and presence of ABA (n = 8 WT; n = 7 cpk3-1cpk6–1; n = 3 cpk3-2cpk6–2 guard cells). GCPs were treated with 50 μM ABA or solvent control (0.1% ethanol) for 2 h prior to establishing gΩ seals. Open and closed symbols indicate −ABA and +ABA, respectively.