Figure 5. Rpe65 Gene Transfer Performed at 1 mo Fails to Protect Cone Photoreceptors from Degeneration.
Rpe65 −/− mice were treated by subretinal injection at age 1 mo with 20 ng of lentiviral vector. Treatment at this age with the LV-RPE65 vector restored ERG response with a threshold response of 3 × 10− 2 cd s/m2 for the b-wave (A), whereas no improvement was observed in the LV-GFP treated group (unpublished data). (B) Counting of PNA-positive and GNAT2-positive cells revealed that Rpe65 gene transfer performed at age 1 mo was unable to protect the cones from degeneration. Data are presented as mean ± SEM.