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Interphase FISH analyses of primary RMS tumor cells with specific BAC and PAC clones confirmed the increased copy number change detected by array-based CGH analysis: ARMS5 tumor cells hybridized with SAS/CDK4 (A) and GLI (B) probes. LAMC2 (C; red) and PAK1 (C; green) signals were detected in ARMS1 cells. (D) Hybridization of ARMS6 cells with the MYCL1 probe.
