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Strains and oligonucleotide primers used in the study.
| Strains | Description |
| ZAP198 | E. coli O157:H7 (Walla3) VT1-VT2- |
| ZAP984 | ZAP198 Δ LEE4 |
| ZAP985 | ZAP784 LEE4 complement |
| Primers | Primer Sequence |
| PIB sp.3 | AGACAAATGGATCTCGTAAGCG |
| PIB sp.5 | GCTGTAACAAGTTGTCTCAGGTGT |
| Plasmids | Description |
| pIB307 | pMAK705 based plasmid for allelic exchange. Temperature sensitive replicon. |
| pAJR162 | pIB307 vector with cloned LEE4 |
