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. 2006 Sep 15;8:87–152. doi: 10.1251/bpo122

Fig. 33. Portion of FF2-6-001 qPCR set-up tool MasterEntrySheet.xls file (Sheet 2) which shows the automatically-calculated master mix set-ups for all final target Sample Plates.

Fig. 33

ABI Cat. No. 4309169, TaqMan® One-Step RT-PCR Master Mix Reagents Kit and the ABI GeneAmp® 5700 Sequence Detection System were used here. All master mix calculation files automatically figure in safe extra preparative amounts so the user will not run short on final volumes or run into bubbles at the bottom of sample tubes.