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. 2006 Oct 6;103(42):15552–15557. doi: 10.1073/pnas.0603781103

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© 2006 by The National Academy of Sciences of the USA

Freely available online through the PNAS open access option.

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Fig. 2. — FLCN–FNIP1 interactions. (A) HA-FLCN was cotransfected with FLAG-FNIP1 into HEK293 cells. Cell lysates were immunoprecipitated (IP) with anti-FLAG or anti-HA antibodies and blotted with both antibodies. Arrows, FLAG-FNIP1; arrowheads, HA-FLCN. (B) HEK293 lysates were immunoprecipitated with anti-FLCN and control IgG or with anti-FNIP1 and control IgG and blotted with anti-FLCN or anti-FNIP1. Arrowheads, endogenous FLCN; arrows, endogenous FNIP1; asterisks, IgG heavy-chain bands. (C) HeLa cells were cotransfected with HA-FNIP1 and FLAG-FLCN. Immunocytochemistry was performed with anti-HA and anti-FLAG antibodies. FNIP1 and FLCN colocalize with a reticular pattern in the cytoplasm. Green, FLAG-FLCN; red, HA-FNIP1.