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. Author manuscript; available in PMC: 2006 Oct 6.
Published in final edited form as: Cancer Res. 2005 Jul 15;65(14):6380–6387. doi: 10.1158/0008-5472.CAN-05-0575

Figure 5.

Figure 5

Figure 5

Figure 5

Figure 5

JNK1 activation has a crucial role in DBPT-induced apoptosis. A, DLD-1 cells transfected with dnJNK1 or dnJNK2 plasmids were analyzed by western blot with anti-HA antibodies. DLD-1 cells stably transfected with dnJNK1, dnJNK2 or an empty vector (control) were treated with 3 μM DBPT for 24 or 48 h. Cell viability was determined by XTT assay (B), and apoptotic ratio was determined by flow cytometry (C). Data represent mean ± SD of three independent experiments. *, P< 0.01, **, P< 0.001 among the indicated groups. D, DLD-1 cells stably transfected with dnJNK1 or dnJNK2 were treated with 3 μM DBPT for 24 and 48 h. Whole-cell lysates were analyzed for indicated antibodies by western blot analysis.