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. Author manuscript; available in PMC: 2007 Jan 1.
Published in final edited form as: Plasmid. 2005 Aug 22;55(1):27–38. doi: 10.1016/j.plasmid.2005.06.002

Figure 1. pFN1-based shuttle plasmids.

Figure 1

A. The first generation pHS17, and second generation pHS23 and pHS30 shuttle plasmids are illustrated schematically with relevant replication origins, genes and restriction endonuclease sites indicated. pHS23 was constructed by deletion of the HpaI fragment of pHS17, resulting in elimination of the pFN1 rlx gene. The pFN1 fragment in pHS23 and pHS30, encoding the fusobacterial replicon, are identical. pHS17 and pHS23 possess the erm cassette and confer macrolide resistance, whereas pHS30 possesses the catP gene and confers resistance to chloramphenicol and thiamphenicol. All sites for the designated restriction endonucleases indicated, and unique sites within a given plasmid are indicated in bold type.