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. Author manuscript; available in PMC: 2007 Jan 1.
Published in final edited form as: Plasmid. 2005 Aug 22;55(1):27–38. doi: 10.1016/j.plasmid.2005.06.002

Table 2.

Transformation of F. nucleatum ATCC 23726 with Shuttle Plasmids of Heterologous Origin1

# of Transformants2
Plasmid DNA Conc. Exp. #1 Exp. #2 Average Average Transformation Efficiency3 Fold Increase in Average # Transformants4
pHS17 1 μg 102 106 104 1.0 X 102
2.5 μg 91 422 257 1.0 X 102
pHS23 1 μg 1,800 10,500 6,150 6.2 X 104 ↑ 59 X
2.5 μg 4,800 41,900 23,350 9.3 X 103 ↑ 91 X
pHS30 1 μg 74,550 224,050 149,300 1.5 X 106 ↑ 1435 X
2.5 μg 179,750 256,600 218,175 0.87 X 106 ↑ 849 X
pHS19 1 μg 0 0 0 0
2.5 μg 0 0 0 0
1

The plasmid DNA used in these studies was isolated from the heterologous host, E. coli DH5α.

2

Electroporation was conducted in two independent experiments with 100 microliters of F. nucleatum ATCC 23726 cells at 2.5 kV and 100 Ohms. Shuttle plasmid DNA of 1 μg was added in a volume ranging from 1.6 to 3.9 λ, and of 2.5 μg the volume ranged from 4.0 to 9.6 λ.

3

Transformation efficiency is defined as the number of transformants/microgram of DNA.

4

Increase in recovery of transformants as compared to the same concentration of pHS17 DNA.