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. Author manuscript; available in PMC: 2006 Oct 6.
Published in final edited form as: Nat Immunol. 2004 Dec 5;6(1):31–41. doi: 10.1038/ni1150

Figure 2.

Figure 2

Contraction and decontraction of the Igk locus. (a) Igk locus (not drawn to scale), indicating the positions of BAC 296M13 (ref. 50) and plasmid IgkC24, which were used to generate the Vκ24 (red) and Cκ (green) probes, respectively. (b) Representative confocal sections through B cells at various stages of development (above images) in which three-dimensional DNA FISH analysis of the Igk locus was done with Vκ24 (red) and Cκ (green) probes. The two Igk alleles of each cell are presented in separate optical sections. Flow cytometry sorting of the developmental stages is in Supplementary Fig. 1 online. (c) Separation of Vκ24 and Cκ gene segments. The distance (in μm) separating the Vκ24 and Cκ segments was evaluated statistically for cells of various developmental stages (horizontal axis). Actual numbers and sample sizes are in Supplementary Table 1 online. Imm.B, immature B cell.