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. Author manuscript; available in PMC: 2006 Oct 6.
Published in final edited form as: Nat Immunol. 2004 Dec 5;6(1):31–41. doi: 10.1038/ni1150

Figure 5.

Figure 5

IL-7 signaling prevents centromeric recruitment of the Igh locus in splenic B cells. (a) Two-color three-dimensional DNA FISH analysis of activated splenic B cells with (+IL-7) or without (Control) IL-7, showing the proximity of the Cγ1 (Igh) and Cκ (Igk) signals (green) with γ-satellite clusters (red). Both Igh or Igk alleles are on the same optical section. (b) Statistical analysis of cells with centromeric association of one Igh or Igk allele in splenic B cells before and after in vitro activation with anti-CD40 in the presence (+IL-7) or absence of IL-7. Actual numbers and sample sizes are in Supplementary Table 4 online.