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. Author manuscript; available in PMC: 2006 Oct 6.
Published in final edited form as: Nat Immunol. 2004 Dec 5;6(1):31–41. doi: 10.1038/ni1150

Figure 7.

Figure 7

Proximal VH-DJH rearrangements in M54 transgenic B cells. IgMa CD19+ B cells expressing the μa transgene were isolated by flow cytometry sorting from the spleens of M54 transgenic mice (Supplementary Fig. 4 online). PCR was used to determine the frequency of the different VH-DJH rearrangements in these transgenic B cells versus Pax5−/− bone marrow pro–B cells and wild-type B220+ splenocytes. Threefold (3×) serial DNA dilutions were analyzed by PCR with VH family–specific forward primers and a JH3 reverse primer12, which was unable to amplify the VHDJH4-rearranged M54 transgene34. Bottom, input DNA was normalized by PCR amplification of an Igh Cμ fragment; far right lane, DNA of stromal ST2 cells (negative control). Numbers along the left margin indicate rearrangements to the JH1, JH2 and JH3 segments. The same result was obtained in two independent experiments.