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Effects of RecA on recA transcription. RT-PCR analysis performed on total RNA extracted from a wild type and a congenic strain containing a recA null mutation [10]. RNA was extracted from exponentially growing cells before or after exposure to sublethal concentration (20 ng/ml) of mitomycin C [10]. The 16S RNA gene was used as a standard to calibrate the amount of RNA to be used with synthetic oligonucleotides amplifying the recA gene.
