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Normalized expression of the MGP gene in old HTM cells. MGP transcription of HTM cells of passage 5 after 1 week (young) and 8 weeks (old) in culture was measured by RQ-RT PCR. Multiplex amplifications were performed at the predetermined exponential 29 cycles and an 18s RNA primer-competimer ratio of 1:9. Expression of MGP was normalized against 18s RNA (mean ± SEM, n = 3 each). Inset: representative 1.5% Tris-borate agarose gel. *P ≤ 0.009.
