Fig. 1.

MIM induced adipogenesis in C3H10T1/2 cells but not in 3T3-L1 cells. Either C3H10T1/2 (Upper) or 3T3-L1 (Lower) cells were grown to confluence. Cells were then exposed to isobutylmethylxanthine and insulin alone (IM, Left) or with DIM (Center) or MIM (Right). After 6–10 days of culture, cells were fixed and stained with Oil-Red-O to identify adipocytes. IM was not sufficient to induce adipogenesis in either cell line. DIM induced adipogenesis in both cell lines. In C3H10T1/2 cells, but not in 3T3-L1 cells, MIM induced adipogenesis.