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. 2006 Oct 12;116(11):2964–2971. doi: 10.1172/JCI28326

Figure 5. Nfkb2–/– thymic stroma are sufficient to induce autoimmunity in thymi-grafted nude mice.

Figure 5

(A and B) Thymic reconstitution was analyzed by counting total cell number and flow cytometry. Numbers represent the percentages within the indicated regions. (C) Real-time PCR analysis of Aire and Ins1 expression in grafted thymi. Values are arbitrary units normalized to Gapdh. Data are mean ± SD for each group. *P < 0.05 versus WT. (D) H&E staining of livers, lungs, pancreata, and salivary glands from nude mice grafted with WT or Nfkb2–/– thymic stroma 12 weeks after thymi grafting. The incidence of infiltration in the specific organs is indicated. (E) Immunofluorescence staining of Rag1–/– tissue sections to test the autoantibodies from the thymi-grafted nude mice. The incidence of infiltration in the specific organs is indicated.