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. 2006 Mar 19;2006(2):16161. doi: 10.1155/MI/2006/16161

Figure 4.

Figure 4

Effects of (a) AG-490 and (b) WHI-P154 on iNOS protein expression in J774 macrophages. Cells were incubated with IFN-γ (5 ng/mL) in the presence or in the absence of the tested compound for 24 hours. Cells were lysed and the protein content of the lysates was measured, and equal amounts of proteins (20 μg) were subjected to immunoblot analysis with an antibody against iNOS. The results are shown as mean ± SEM (n = 6 for AG-490 and n = 4 for WHI-P154), **P < .01 when compared to cells treated with IFN-γ alone.