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Effect of enhanced affinity mutations on binding of IL-2 to IL-2Rα and SP4206
| Mutant | EC50, nM |
|
|---|---|---|
| IL-2Rα | SP4206 | |
| WT | 10.5 (1) | 68.8 (1) |
| V69A/P65A/Q74P | 1.1 (10) | >100,000 |
| K35L/M39V | 1.9 (5) | 80.1 (1) |
| P65A | 5.2 (2) | 117.0 (2) |
| V69A | 2.0 (5) | 10.4 (7) |
The approximate fold improvement in affinity of each variant versus WT IL-2 for either IL-2Rα or SP4206 is shown in parentheses. Each result is the average of three independent experiments. Experimental details are outlined in Materials and Methods.
