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. 2003 Nov 17;198(10):1583–1593. doi: 10.1084/jem.20031051

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Copyright © 2003, The Rockefeller University Press

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Figure 4. — Memory OT-I T cells preferentially relocate to peripheral organs and respond to IL-12 and IL-18 by secreting IFN-γ. (A) 10⁷ nylon wool-purified OT-I splenocytes were transferred into B6.Thy1.1 hosts. (B and C) 3 × 10⁶ nylon wool-purified OT-I T cells were transferred into B6.Thy1.1 hosts, challenged with ∼2,000 LM/OVA, and then rested for >4 wk. Graphs representing the percentage of Thy1.2⁺ (OT-I) cells out of the total CD8⁺ population within the indicated organs for the naive OT-I T cells (A) and the memory OT-I T cells (B) are shown. (C) After overnight stimulation with either 10 nM SIINFEKL or a combination of IL-12 and IL-18, the cells isolated from the indicated organs were incubated for 4 h with GolgiPlug™ and then stained for CD8, Thy1.2, and intracellular IFN-γ. Three mice were averaged per group, and the data are representative of three independent experiments.