Figure 3.

Lesions from ApoE^−/− mice contain nitrated PGHS-1 that is reduced in ApoE^−/−iNOS^−/− mice. Aortic tissue from ApoE^−/− and ApoE^−/−iNOS^−/− mice fed a Western diet for 6 months was dissected into portions representing the plaques (P) and noninvolved surrounding medial tissue (M). The tissue was homogenized as described in the Materials and Methods. Proteins containing 3-nitrotyrosine were immunoprecipitated using a polyclonal 3-nitrotyrosine antibody and then immunoblotted with a PGHS-1 monoclonal antibody. This experiment has been reproduced three times using three different pools of tissue. Each tissue pool is from ∼10 ApoE^−/− and ∼10 ApoE^−/−iNOS^−/− mice.