Figure 1.

GAS5 and CHOP mRNA is time dependently expressed in X/XO and H₂O₂-treated differentiated mouse podocytes. Time course for the induction of GAS5 and CHOP mRNA by X/XO and H₂O₂. Gas5 and CHOP mRNA induction was studied in control podocytes or in podocytes that have been incubated with X/XO (50 μmol/L/50 mU/ml) (A) or H₂O₂ (250 μmol/L) (B) for the indicated times. Experiments were performed by using RT or no RT in each set-up (RT− not shown). The densitometric analysis of mRNA expression profiles for CHOP was normalized with GAPDH mRNA expression. Studies were performed with primers derived from published mouse cDNA sequences resulting in a 379-bp fragment for CHOP and two 344- and 301-bp fragments for GAS5. Sequence analysis of the resulting amplification products revealed sequence identity for the amplified fragments with published sequences (n = 3 to 15, values are means ± SEM, *P < 0.05 versus control, t-test).