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. 2006 Jan;168(1):20–32. doi: 10.2353/ajpath.2006.040774

Figure 4.

Figure 4

NADPH-mediated superoxide anion production in control cells and in CHOP-overexpressing podocytes. Top left: Expression levels for CHOP in podocytes overexpressing CHOP and control cells transfected with vector only. Top right: Statistical analysis (n = 5, values are means ± SEM, *P < 0.05 versus control, t-test). Middle left: Recording of the time course and magnitude of NADPH-oxidase activation in control cells and CHOP-overexpressing podocytes. Middle right: Summary of the experiments. O2− generation by NADPH-oxidase activity was calculated by integrating the total counts during the first 15 minutes of stimulation. Values are expressed as nmol O2− generated per mg cellular protein per minute (n = 6, values are means ± SEM, *P < 0.05 versus control, t-test). Bottom: CHOP down-regulates GAS5 mRNA expression in CHOP-overexpressing cells (CHOP) compared to vector-only transfected cells (Co). GAPDH expression was not different in both cell lines.