Figure 4-6715.

CBD reduces VEGF expression in experimental diabetes. The distribution and quantification of VEGF in retinal sections were analyzed by immunolabeling using VEGF antibody followed by Oregon Green-conjugated secondary antibody. A: Representative images showing the fluorescence distribution in different retinal layers; the ganglion cell layer (GCL), the inner nuclear layer (INL), the outer nuclear layer (ONL), and the retinal pigment epithelium (RPE) after 2 or 4 weeks of induced diabetes. B: Morphometric analysis shows that diabetic retinas have significant increases of VEGF expression (twofold, 1.7-fold) after 2 and 4 weeks of induced diabetes, respectively, compared to untreated controls. Treatment with CBD (10 mg/kg every 2 days) significantly blocked the increase in VEGF expression. CBD treatment did not alter amount or distribution of VEGF in the treated controls. Data shown is the mean ± SEM of six animals in each group (*P < 0.05). Original magnifications, ×200.