Table 1-6704.
Pancreatic SSAT Activities, Polyamine Pools, α-Amylase Activities, and Histology in Nontransgenic Rats after Treatment with l-Arginine or Cerulein with or without Pretreatment with MeSpd
| Group (number of animals) | SSAT (pmol/mg/10 minutes) | Putrescine (pmol/mg tissue weight) | Spermidine (pmol/mg tissue weight) | Spermine (pmol/mg tissue weight) | MeSpd (pmol/mg tissue weight) | α -Amylase (U/L) | Histology‡ |
|---|---|---|---|---|---|---|---|
| Control (9) | 3.6 ± 0.8 | 7 ± 9 | 4405 ± 1708 | 862 ± 364 | 1913 ± 462 | Normal | |
| l-arginine (8) | 10.5 ± 6.4* | 293 ± 340 | 2138 ± 1297† | 406 ± 170† | 20,785 ± 8681† | Moderate to severe | |
| l-arginine + | 13.7 ± 7.0† | 492 ± 353* | 3276 ± 1420† | 584 ± 183* | 1669 ± 705 | 5221 ± 3838 | Normal to mild |
| MeSpd (11) | |||||||
| Control (5) | 4.4 ± 1.6 | 32 ± 3 | 4100 ± 320 | 750 ± 260 | 2650 ± 210 | Normal | |
| Cerulein (6) | 31.9 ± 4.7† | 185 ± 47† | 1750 ± 230† | 340 ± 63† | 69,850 ± 8040† | Mild to moderate | |
| Cerulein + | 34.9 ± 5.2† | 197 ± 56† | 1770 ± 224† | 320 ± 25† | ND | 80,310 ± 15,230† | Mild to moderate |
| MeSpd (5) |
The rats received l-arginine (2.5g/kg i.p.) 24 hours before sacrifice or cerulein (50 μg/kg i.p. in seven hourly injections) before being sacrificed 1 hour after the last cerulein injection. MeSpd (50 mg/kg i.p.) was administered as two doses 20 hours and 4 hours before l-arginine or cerulein. The data are expressed as means ± SD. ND, not detectable. Numbers of animals per group are given in parentheses after each group definition.
*
P < 0.05 and
†
P < 0.01 as compared with untreated control animals.
‡
For definition of evaluation, see Materials and Methods.