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. 2006 Feb;8(1):89–96. doi: 10.2353/jmoldx.2006.050049

Figure 3.

Figure 3

Limit of detection (LOD) for B. anthracis Ames genomic DNA. Representative data determining LOD using 10-fold dilution series from 1 pg to 1 fg of B. anthracis Ames total genomic DNA in duplicate. A: Real-time PCR data of decreasing relative fluorescence units (RFU) versus PCR cycle number. B: Postamplification thermal melt analysis of PCR products as the negative first derivative of RFU with respect to temperature (−d(RFU)/dT) versus temperature in °C. Data are from the cya:capB:IPC multiplex. LOD for cya primer set was 10 fg or ∼2 copies.