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Positive Control Testing Results from the paga:capb:IPC and cya:capb:IPC MultiCode-RTx B. anthracis Assays
|
pagA:capB:IPC
|
cya:capB:IPC
|
|||||||
|---|---|---|---|---|---|---|---|---|
|
pagA - F1 - FAM
|
capB - F2 - HEX
|
cya - F1 - FAM
|
capB - F2 - HEX
|
|||||
| Ct | Tm | Ct | Tm | Ct | Tm | Ct | Tm | |
| Mean | 37.4 | 79.2 | 38.3 | 81.8 | 31.8 | 76.2 | 35.4 | 80.1 |
| SD | 1.7 | 0.2 | 1.9 | 0.1 | 0.6 | 0.3 | 1.1 | 0.4 |
| %CV | 4.5 | 0.2 | 5.0 | 0.1 | 1.9 | 0.4 | 3.0 | 0.5 |
Eight reactions of each assay were run using 1 pg of B. anthracis Ames DNA. The mean Ct, Tm, SD, and %CV are tabulated.
