TABLE 2.
Antibody titers (assessed by IFA against sporozoites) from Aotus monkey sera inmunized with CSP, TRAP, and LSA-1 peptidesa
| Protein | Original peptide | Peptide no.b | Sequence | Titer
|
||||
|---|---|---|---|---|---|---|---|---|
| PI | I20 | II10 | II15 | II30 | ||||
| CS | 4383 | 24258 | CGKKISFSLGEKVIGNNGC | 0 | 0 | 1/640 | ND | 1/320 |
| 4389-4390 | 13516 | CGHNMPNDPIFMSDENANANSAVKNGC | 0 | 0 | ND | 3/320 | 3/640 | |
| 4389-4390 | 13526 | CGHNMPNDPMMMSDENANGMGTVKNGC | 0 | 0 | ND | 1/320 | 1/1,280 | |
| TRAP | 3289 | 24246 | CGSPTSVTVGKGAFSFKREGC | 0 | 1/160 | 3/1,280 | 1/1,280 | 1/1,280 |
| 3347 | 24254 | CGGAATPYSGEPSPFDEVLGEEGC | 0 | 3/640 | 4/640 | 3/640 | 3/1,280 | |
| 3287-3289 | 24242 | CGVWDEWSPVSTAVGMGTRSRKGC | 0 | 5/1,280 | 5/1,280 | 2/1,280 | 2/1,280 | |
| 3277-3279 | 24238 | CGVAFNRFHVGTHPAPGKTNLYGC | 0 | 3/640 | 3/320 | 4/640 | 3/640 | |
| 3285-3287 | 24240 | CGTVEVEMTASTGVWNMWSPGC | 0 | 3/320 | 1/320 | 1/320 | 1/320 | |
| LSA-1 | 20630 | 24282 | CGINGKNIKNAEKPMIIKSNLRGC | 0 | 6/640 | 3/640 | 1/320 | 1/320 |
a
The analogue peptides were injected on days 0 (I) and 20 (II), and bleeding was carried out at 0 days. (PI) and 20 days after the first immunization (I20) and 10, 15, and 30 days after the second immunization (II10, II15, and II30, respectively). The peptides were tested in groups of five to nine animals (depending on availability). The peptides induced antibodies in Aotus monkeys after the second immunization, which recognized sporozoites by IFA. Underlined letters are the amino acids changed from the original sequence.
b
Designated by the synthesis group of the Fundacion Instituto de Immunología de Colombia (FIDIC).