TABLE 2.
α-specific genes are not required for HML repression as a donor in MATα cells
| Strain | Genotype | % HML usagea |
|---|---|---|
| Cwu150 | MATa | 75 |
| Cwu128 | MATa GT→TG AC→TA (Matα2 sites A and B) | 75 |
| KS338 | MATa 4A | 65 |
| Cwu151 | MATα | 15 |
| Cwu134 | MATα GT→TG AC→TA (Matα2 sites A and B) | 55 |
| KS345 | MATα 4A | 50 |
| ECY119 | MATα1::KanMX4 | 20 |
| ECY120 | MATα1::KanMX4 GT→TG AC→TA (Matα2 sites A and B) | 48 |
| ECY121 | MATα1::KanMX4 4A | 47 |
a
HML usage has been determined in MATa strains carrying HMLα and HMRα-BamHI, in MATα-BamHI strains carrying HMLα and HMRa, or in MATα1::KanMX4 strains carrying HMLα and HMRa. Genomic DNA extracted from an entire population of cells in which mating-type switching has been induced was cut by BamHI and StyI and probed with a MAT distal fragment.