FIG. 4.
Synergistic NF-κB activation of CARMA1 with CaMKII upstream of IKK. (A) Jurkat cells were electroporated with NF-κB reporter, pRL-TK construct, 2 μg of CaMKII1-290, and 2 μg of Flag-CARMA1 construct. One day later, luciferase activities were determined. (B) Jurkat cells were electroporated with NF-κB reporter, pRL-TK construct, 2 μg of the IKKβ dominant-negative (d/n) mutant (with S177A and S181A mutations), and CaMKII1-290 or Flag-CARMA1. One day later, luciferase activities were determined. (C) Jurkat cells were transfected as described for panel B except that CaMKII1-290 and CARMA1 were cotransfected. Western blot analysis using cell lysates was performed with rabbit anti-CaMKII antibody or monoclonal anti-Flag antibody (lower panels). Three independent experiments were performed in duplicate. Bars represent standard errors.