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. 2006 Aug;26(15):5688–5697. doi: 10.1128/MCB.00779-06

FIG. 3.

FIG. 3.

Comparison of ER chaperone protein expression levels in wild-type and Grp78+/− mice. (A) Lysates from livers of WT (+/+) adult mice and Grp78+/− siblings were subjected to Western blot with anti-KDEL (detects GRP94, GRP78, and PDI), anticalnexin (CNX), anti-calreticulin (CRT), and anti-β-actin antibodies. (B) Quantification of Western blot analyses performed in panel A for four pairs of mice with Quantity One software (Bio-Rad). β-Actin was used as a protein loading control. For each protein, the WT level was set as 1. The standard deviations are shown. The Student's t test was performed to determine the statistical significance between the wild-type (+/+) and heterozygous (+/−) levels (* denotes a P value of <0.05; ** denotes a P value of <0.01). (C) Lysates from E10.5 embryos of WT mice and Grp78+/− siblings were subjected to Western blot analysis with anti-KDEL and anti-β-actin antibodies. (D) Quantification of Western blots in panel C.