FIG. 7.

Recruitment of RNA polymerase II to the chromatin of the 5′ HS cluster and in the Kit promoter region. A schematic representation of the Kit locus and the transgene constructs is shown at the top. The positions of the different PCR amplification units A2 (−247 to +63 of GFP), A4 (−247 to +37), and A5 (−247 to +3) and P1, P3, P4, P6, P7, P8, and P9 used for quantitation are indicated by horizontal bars. Formaldehyde-cross-linked chromatin obtained from WT (+/+) BMMC, W^sh/W^sh BMMC, 32D cells, and BMMC derived from BAC200-Kit-GFP (T20)/W^sh/W^sh, BAC30-Kit-GFP (T51)/W^sh/W^sh, and BAC200-Δ5′HS-Kit-GFP (T78) mice was immunoprecipitated with antibodies against RNA polymerase II, and the amount of immunoprecipitated and input DNA was assayed by semiquantitative PCR. The ratios of signals of bound versus input chromatin were determined with a phosphorimager and are represented in the histogram. The error bars represent the standard deviations from three independent ChIP experiments.