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Both Aur1 and Stt4 function in sphingolipid metabolism. (A) Tenfold serial dilutions of the indicated cells were spotted on YPD at 26°C or 30°C for 2 days. (B) stt4ts, stt4ts/aur1ts, and aur1ts cells were incubated for 90 min at the indicated temperatures. Cells were fixed and stained with rhodamine-phalloidin and visualized by fluorescence microscopy. DIC, differential interference contrast.
