FIG. 5.

PPAR subtype-specific activation of endogenous genes in NIH 3T3 cells and MEFs. The PPARs activate endogenous target genes in a highly subtype-specific manner. NIH-CAR cells (A) and MEFs (B) were transduced with AdHA-PPARα, -γ2, or -β/δ (55 PFU/cell) in the presence of 30 μM WY14.643, 1 μM rosiglitazone/BRL49653, or 1 μM L-165041, respectively. RNA was purified 0, 4, 8, and 12 h after transduction, and expression of target genes was determined by real-time PCR, normalized to TFIIB expression and shown relative to the A-FABP/TFIIB level of nontransduced cells. A subset of genes representing different types of target genes is shown. The increase in induction (n-fold) of each gene at the 12-h time point is indicated. For the complete set of target genes, please refer to http://www.sdu.dk/Nat/bmb/faculty/pubs/MCB06_supp_data.html. Results are representative of at least four independent experiments.