FIG. 7.

The role of agonists in PPAR subtype-specific activation of endogenous target genes. (A) Activation of PPAR target genes in the presence and absence of agonists. NIH-CAR cells were transduced and treated as in described in the legend of Fig. 5A, except that the experiment was carried out in the presence as well as the absence of agonists. RNA was harvested 12 h after transduction, and expression of target genes was determined by real-time PCR, normalized to TFIIB and shown relative to the A-FABP/TFIIB level of nontransduced cells. Results are representative of at least four independent experiments. (B) Relative occupancy of HA-PPARs and RXR on PPAR target promoters in the presence and absence of agonists. NIH-CAR cells were transduced and treated as described in the legend of Fig. 6B except that the experiment was carried out in the presence as well as the absence of agonists. Relative occupancy at the indicated target genes is indicated. Results are representative of at least three independent experiments.