FIG. 5.

In vitro translation of fLuc-8R does not respond to alterations in the levels or availability of the DICE-binding proteins αCP and hnRNP K. (A) Translation of fLuc-8R mRNA is not repressed by the addition of recombinant αCP1, recombinant hnRNP K, or the combined addition of recombinant αCP1 and hnRNP K (left panel) to the translation extract. The translations of the fLuc-8R and fLuc-8Rm mRNAs were carried out in reticulocyte extracts supplemented with increasing amounts of recombinant αCP1, hnRNP K, or αCP1 plus hnRNP K as indicated by the wedge below the histogram. The data represent four independent studies; means and standard deviations are shown for each of the translation conditions. (B) Blockade of αCP activity with dC₁₇ fails to alter translational activity of fLuc-8R mRNA. Addition of dC₁₇ to the RRL markedly inhibits translation of fLuc under the control of the αCP-dependent poliovirus IRES function (IRES-fLuc). There is no apparent impact of the same decoy effect on the DICE-linked reporter fLuc-8R. The data represent the results from three independent studies; means and standard deviations are shown for each of the translation conditions.