FIG. 7.

Stabilization of mRNA by DICE-2R is sensitive to nuclear αCP decoys. The α^2R or α^2Rm expression vector was separately transfected into MEL/tTA cells along with plasmids encoding the nuclear decoy U1-R7α1, its empty framework U1 RNA, the cytoplasmic decoy VA1-R7α1, or its corresponding empty framework VA1 RNA. The half-life of α^2R or α^2Rm mRNA under each treatment was derived from the decay curve plotted from the RPA data. The computed half-life is shown in the box, and representative RPA gels of 8-h chase studies are displayed below each of the corresponding graphs. The data represent results from five to seven independent 8-h or 16-h chase studies.