FIG. 3.

Prolactin affects the stability of NF1-C2 proteins through Jak2. (A) Nuclear extracts were prepared from double-thymidine-blocked cells treated with (+) or without (−) prolactin for 4 h and subjected to Western blot analysis. The filter was first incubated with the NF1-C2-specific antibody and then stripped and reincubated with an HDAC-1 antibody. (B) Nuclear extracts were prepared from G₁/S-synchronized as well as unsynchronized HC11 cells treated with different inhibitors for 20 min as indicated in the figure. Where indicated, prolactin was added to the cells for 20 min. The different blots were first incubated with the NF1-C2-specific antibody and then stripped and reincubated with the HDAC-1 antibody.