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. 2006 Aug;26(15):5663–5674. doi: 10.1128/MCB.02095-05

FIG. 7.

FIG. 7.

Nuclear Jak2 affects the expression of p53 and CEL genes through NF1-C2. (A) Western blot analysis with nuclear extracts from HC11 cells grown to different degrees of confluence (stages I and II). Stage I is less confluent than stage II. (B) The endogenous mRNA expression levels of the p53 gene in HC11 cells grown to different degrees of confluence (stages I and II) were assayed. (C) Luciferase activity levels were measured in HC11 cells stably transfected with either the mCEL-1831Luc or the mCEL-1831NF1:1mutLuc construct and grown to different degrees of confluence as indicated. The relative luciferase activity levels were normalized to the total protein concentration of each extract. Data represent means from three independent experiments. To illustrate the proportional difference between the two promoter constructs, the average luciferase activity level of the wild-type (wt) construct from each stage was divided by the average activity level of the mutant (mut) construct from each stage.