FIG. 8.

The decreased level of tyrosine-phosphorylated NF1-C2 proteins in the nucleus at lactation correlates with a decrease of active Jak2 in the nucleus at this stage. (A) Nuclear extracts from mammary glands at P16, L1, and L6 were subjected to Western blot analysis. The filter was incubated with the anti-NF1-C2 antibody. (B) Upper panel: Western blot analysis of nuclear extract from P16 and L6. The blot was incubated with anti-Jak2 antibody. Lower panel: 150-μg portions of nuclear extracts from P16 and L6 were immunoprecipitated (IP) with anti-pY antibody and subsequently analyzed by Western blotting (WB) with anti-Jak2 antibody.