FIG. 7.

Defective nasal development and cleft palate in Crk^−/− embryos. E15.5 Crk^−/− and wild-type littermates were fixed and embedded in paraffin, and coronal and sagittal sections were obtained. Paraffin sections were stained with hematoxylin and eosin, and matching images of the nasal parts were taken. (A to F) Panels A, B, and C are anatomically matching coronal sections that are anterior to panels D, E, and F, respectively. (G and H) High-magnification views of the edema formed in panels C and E, respectively. (I to N) Panels I, J, and K are anatomically matching sagittal sections, and their high-magnification views of the nasopharynx are shown in panels L, M, and N, respectively. Nasal cavity (nc), nasal septum (ns), cartilage primordium of nasal septum (cn), edema (*), palate (p), cleft palate (cp), nasopharynx (np), and oropharynx (op) are marked. Bar, 250 μm.