FIG. 6.

Ro52 is required for S-phase progression. (A) Aliquots of HeLa cells transfected with the indicated siRNAs, synchronized by growth in nocodazole, and then released for the indicated periods of time were analyzed by FACS. The y axis indicates cell number and the x axis indicates DNA content as determined by propidium iodide staining. Exp., exponentially growing HeLa cells. (B) Percentage of cells in S phase analyzed as described for panel A. (C) Aliquots of cells shown in panel A were processed for immunoblotting with antibodies against indicated proteins. ctr., control.