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. 2006 Aug;26(16):6223–6238. doi: 10.1128/MCB.02324-05

FIG. 5.

FIG. 5.

IFM analysis of nucleolus in yeast strains carrying expanded rDNA* repeats at ectopic sites. (A) Yeast strains NOY388 (wild type [WT]), NOY2029 (rDNA* array at the native site, “Chr. XII”), NOY2030 (rDNA* array near CEN), NOY2031 (rDNA* array at mid-V-R), and NOY2032 (rDNA* array near TEL VI-R) were analyzed for the A190 subunit of Pol I and for Nop1 using IFM. DNA was stained with DAPI. Images of A190 (green), Nop1 (red), and DAPI (blue) were overlaid. Individual images are shown in black and white, and the overlaid one is shown in color (“merge”). (B) IFM analysis of location of the nucleoli relative to the location of SPB. Strains carrying expanded rDNA* analyzed in panel A were examined. The YOL1/34 monoclonal antibody to yeast tubulin showed the area to be slightly larger than the SPB in interphase cells (green), and antibodies to A190 revealed the nucleolus containing Pol I (red). Overlays of these two images and DAPI-stained DNA (blue) are shown in color. Arrows indicate the positions of contact of the nucleolus with CEN5 that is close to SPB.