TABLE 1.
Plasmids and yeast strains used in this study
| Plasmid or strain | Description |
|---|---|
| Plasmids | |
| pNOY130 | High-copy-number plasmid carrying GAL7-35S rDNA 5S rDNA URA3 2μ amp (54) |
| pNOY3286 | A derivative of pBluescript SK(+) carrying a single rDNA* (hyg1, 26-bp tag) copy and the nonyeast DNA (N) and HIS3 |
| pNOY3289 | A derivative of pUC18 carrying the DNA fragment used for integration of a single rDNA* copy at the site in the middle of chromosome V-R |
| pNOY3290 | A derivative of pUC18 carrying the DNA fragment used for integration of a single rDNA* copy at the site near TEL VI-R |
| pNOY3291 | A derivative of pUC18 carrying the DNA fragment used for integration of a single rDNA* copy at the site near CEN5 |
| pNOY3292 | A derivative of pUC18 carrying the DNA fragment used for a single integration of rDNA* copy at the site corresponding to the native RDN1 site in rdnΔΔ strains (see Fig. 1B) |
| pNOY3293 | A derivative of pBluescript SK(+) carrying the DNA fragment (∼13-kb SpeI-SalI fragment; Fig. 1C) used for integration of a second rDNA* copy and subsequent rDNA* repeat expansion |
| pNOY3300 | A derivative of pNOY3291 with rDNA* transcribing in the opposite direction (see Fig. 3A) |
| pNOY3313 | A derivative of pNOY3291 with the E element deleted (see Fig. 3A) |
| pNOY3318 | A derivative of pNOY3289 with rDNA* transcribing in the opposite direction |
| pNOY3319 | A derivative of pNOY3290 with rDNA* transcribing in the opposite direction |
| Strains | |
| NOY388 | MATaade2-1 ura3-1 trp1-1 leu2-3,112 his3-11,15 can1-100 |
| NOY396 | MATα ade2-1 ura3-1 trp1-1 leu2-3,112 his3-11,15 can1-100 |
| NOY886 | MATα rpa135Δ-LEU2 ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 fob1Δ::HIS3, pNOY117 [CEN RPA135 TRP1]; rDNA copy no. ∼40 (12) |
| NOY984 | MATα ade2-1 ura3-1 trp1-1 leu2-3,112 his3-11,15 can1-100 rdnΔΔ::hisG, pNOY130 |
| NOY986 | MATaade2-1 ura3-1 trp1-1 leu2-3,112 his3-11,15 can1-100 rdnΔΔ::hisG, pNOY130 |
| NOY987 | MATα ade2-1 ura3-1 trp1-1 leu2-3,112 his3-11,15 can1-100rdnΔΔ::hisG, pNOY130, single rDNA* repeat near CEN5 |
| NOY988 | MATα ade2-1 ura3-1 trp1-1 leu2-3,112 his3-11,15 can1-100rdnΔΔ::hisG, pNOY130, single rDNA* at mid-V-R |
| NOY1071 | MATα ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 fob1Δ::HIS3, rDNA copy no. ∼25 (7) |
| NOY2029 | MATaade2-1 ura3-1 trp1-1 leu2-3,112 his3-11,15 can1-100, rDNA* array substituting for RDN1 |
| NOY2030 | MATα ade2-1 ura3-1 trp1-1 leu2-3,112 his3-11,15 can1-100rdnΔΔ::hisG, rDNA* array near CEN5 |
| NOY2031 | MATα ade2-1 ura3-1 trp1-1 leu2-3,112 his3-11,15 can1-100rdnΔΔ::hisG, rDNA* array at mid-V-R |
| NOY2032 | MATaade2-1 ura3-1 trp1-1 leu2-3,112 his3-11,15 can1-100, rdnΔΔ::hisG, rDNA* array near TEL VI-R |
| NOY2039 | MATa/MATα ade2-1/ade2-1 ura3-1/ura3-1 trp1-1/trp1-1 leu2-3,112/leu2-3,112 his3-11,15/his3-11,15 can1-100/can1-100 RDN1/rDNA* array |
| NOY2040 | Same as NOY2039, but RDN1/rdnΔΔ and rDNA* array near CEN5 on one of two chromosome Vs |
| NOY2041 | Same as NOY2039, but RDN1/rdnΔΔ and rDNA* array at mid-V-R on one of two chromosome Vs |
| NOY2042 | Same as NOY2039, but RDN1/rdnΔΔ and rDNA* array near TEL VI-R on one of two chromosome VIs |
| NOY2047 | MATaade2-1 ura3-1 trp1-1 leu2-3,112 his3-11,15 can1-100rdnΔΔ::hisG, pNOY130, single rDNA* repeat near TEL VI-R |
| NOY2048 | MATaade2-1 ura3-1 trp1-1 leu2-3,112 his3-11,15 can1-100, single rDNA* repeat substituting for RDN1, pNOY130 |
| NOY2050 | Same as NOY388, but carries a single rDNA* near CEN5 |
| NOY2051 | Same as NOY388, but carries a single rDNA* at mid-V-R |
| NOY2052 | Same as NOY388, but carries a single rDNA* near TEL VI-R |
| NOY2053 | MATaade2-1 ura3-1 trp1-1 leu2-3,112 his3-11,15 can1-100, RDN1 and rDNA* array near CEN5 |
| NOY2054 | MAT? ade2-1 ura3-1 trp1-1 leu2-3,112 his3-11,15 can1-100, RDN1 and rDNA* array at mid-V-R |
| NOY2055 | MAT? ade2-1 ura3-1 trp1-1 leu2-3,112 his3-11,15 can1-100RDN1 and rDNA* array near TEL VI-R |
| NOY2056 | MATa/MATα ade2-1/ade2-1 ura3-1/ura3-1 his3-11,15/his3-11,15 trp1-1/trp1-1 leu2-3,112/leu2-3,112 can1-100/can1-100 rdnΔΔ::rDNA* (a single repeat)/RDN1 |
| NOY2063 | Same as NOY886, but fob1Δ::hisG instead of fob1Δ::HIS3 |
| NOY2064 | Same as from NOY2063, but carries a single rDNA* near CEN5 (derived from pNOY3291) |
| NOY2068 | Same as NOY1071, but fob1Δ::hisG instead of fob1Δ::HIS3 |
| NOY2146 | Same as NOY2064, but a single rDNA* near CEN5 is in the opposite orientation (derived from pNOY3300) |
| NOY2147 | Same as NOY2064, but a single rDNA* near CEN5 carries ΔE deletion (derived from pNOY3313) |
| NOY2148 | Same as NOY2063, but carries a single rDNA* copy within rDNA repeats at RDN1 (integration carried out using pNOY3286 digested with MluI, which gives a single cut within the 25S rRNA coding region) |
| NOY2149 | Same as NOY2063, but carries a single rDNA* copy at mid-V-R (derived from pNOY3289) |
| NOY2150 | Same as NOY2149, but a single rDNA* copy at mid-V-R is in the opposite orientation (derived from pNOY3318) |
| NOY2151 | Same as NOY2063, but carries a single rDNA* copy near TEL VI-R (derived from pNOY3290) |
| NOY2152 | Same as NOY2151, but a single rDNA* copy near TEL VI-R is in the opposite orientation (derived from pNOY3319) |
| NOY2158 | Same as NOY2159, but carries a second rDNA* near CEN5 (derived from pNOY3296) |
| NOY2159 | Same as NOY2068, but carries a single rDNA* near CEN5 (derived from pNOY3291) |